| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.086251 |
| Chromosome: | chromosome 8 |
| Location: | 3725414 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g377050 | (1 of 1) 4.6.1.14 - Glycosylphosphatidylinositol diacylglycerol-lyase / VSG-lipase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGATCGGCTGGTCCACGGCGAAATCACC |
| Internal bar code: | TACGTGCACCAGGCTTCAATGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 364 |
| LEAP-Seq percent confirming: | 99.6095 |
| LEAP-Seq n confirming: | 7652 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCGTACGAGACGTGCTGAG |
| Suggested primer 2: | TGGTCAGCTATCGTCACTGC |