| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.086251 |
| Chromosome: | chromosome 8 |
| Location: | 3725416 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g377050 | (1 of 1) 4.6.1.14 - Glycosylphosphatidylinositol diacylglycerol-lyase / VSG-lipase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGAAATGTGGAGCCGGCCATAGTTCTTG |
| Internal bar code: | GCCTGATACTAATGAGGCGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 828 |
| LEAP-Seq percent confirming: | 99.6714 |
| LEAP-Seq n confirming: | 910 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCGTACGAGACGTGCTGAG |
| Suggested primer 2: | TGGTCAGCTATCGTCACTGC |