Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.086299 |
Chromosome: | chromosome_5 |
Location: | 2244001 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre05.g234662 | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TCCATTCATAAATTGCAACGGCGCTCCATA |
Internal bar code: | CAGCCCTTATCAAAACGTATTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 845 |
LEAP-Seq percent confirming: | 99.7543 |
LEAP-Seq n confirming: | 1218 |
LEAP-Seq n nonconfirming: | 3 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AGGATGGCATTACCACTTGC |
Suggested primer 2: | AACCAATTGCCTTTTGTTGC |