| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.086303 |
| Chromosome: | chromosome 3 |
| Location: | 423068 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g144967 | (1 of 1) K17681 - ATPase family AAA domain-containing protein 3A/B (ATAD3A_B) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTTCTGGAGCTGCTCGCACACATAGCGG |
| Internal bar code: | CCAAGGCATGTAACCCTGCTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 383 |
| LEAP-Seq percent confirming: | 98.6446 |
| LEAP-Seq n confirming: | 1965 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGCAACAACTGCTGAAGTA |
| Suggested primer 2: | GGAGAAGGGAAGAGTGGACC |