| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.086312 |
| Chromosome: | chromosome 6 |
| Location: | 100988 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g249700 | (1 of 1) IPR003690//IPR017956//IPR020478 - Mitochodrial transcription termination factor // AT hook, DNA-binding motif // AT hook-like | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGCGATGTGTCATGTGTCAAGGATCATG |
| Internal bar code: | ACGGGCGGAAAGGGACTGCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 176 |
| LEAP-Seq percent confirming: | 74.5819 |
| LEAP-Seq n confirming: | 669 |
| LEAP-Seq n nonconfirming: | 228 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTAGCTGTGGACGCGTATC |
| Suggested primer 2: | TGGACAGTGTATCTGCAGCC |