Insertion junction: LMJ.RY0402.086343_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.086343
Chromosome:chromosome 15
Location:49333
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre15.g634600 (1 of 1) PF12796//PF13637//PF13857 - Ankyrin repeats (3 copies) (Ank_2) // Ankyrin repeats (many copies) (Ank_4) // Ankyrin repeats (many copies) (Ank_5) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGAGGAGGAGGAGGAAGACGGCGAGGCAA
Internal bar code:GGCGGTTTAATTGCATACGCCA

Confirmation - LEAP-Seq

LEAP-Seq distance:46
LEAP-Seq percent confirming:94.1176
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:ATACCTGCCGACATTCTTGC
Suggested primer 2:TTGATCTCTACCCGCATTCC