| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.086353 |
| Chromosome: | chromosome 1 |
| Location: | 2372974 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g013000 | (1 of 1) 2.7.7.31 - DNA nucleotidylexotransferase / Terminal transferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGAGGCGGCGGCGGCAGCGGGCCGCGAC |
| Internal bar code: | GATGTCTCCGACTGGTCCTCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 303 |
| LEAP-Seq percent confirming: | 77.9141 |
| LEAP-Seq n confirming: | 127 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCACAACCGTACGACACAA |
| Suggested primer 2: | CGGTGTGTGTGTGTGTGTGT |