| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.086353 |
| Chromosome: | chromosome_10 |
| Location: | 3876347 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g447700 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCAACAGCTCGAGCCGGCCATTGGCGTCCG |
| Internal bar code: | GTTGGTCCATGGCACTGTAATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 171 |
| LEAP-Seq percent confirming: | 97.7314 |
| LEAP-Seq n confirming: | 1077 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGCGTATGCTTTCGACTG |
| Suggested primer 2: | TGCGCTCATAAAGGGAAGTC |