| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.086354 |
| Chromosome: | chromosome 4 |
| Location: | 3177390 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g226150 | AOC1 | (1 of 4) PTHR11785:SF367 - AMINO-ACID PERMEASE BAT1; Amino acid carrier | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGCCACGAATTGCATGAATCTTGCCTTG |
| Internal bar code: | AAGATGATAGGGGCTCCAGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1 |
| LEAP-Seq percent confirming: | 83.8184 |
| LEAP-Seq n confirming: | 6998 |
| LEAP-Seq n nonconfirming: | 1351 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGTACGACAGCCCCACTAC |
| Suggested primer 2: | CTCATTCTTCTGGCACGTCA |