Insertion junction: LMJ.RY0402.086371_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.086371
Chromosome:scaffold 19
Location:29071
Confidence (%):73
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGAACAAAACCTTGGGGGGCCATCCTGAT
Internal bar code:TAGTTGTTACTAAAGAGGCAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:612
LEAP-Seq percent confirming:99.095
LEAP-Seq n confirming:1314
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:GAAGTCCCGTAGAGTGCAGC
Suggested primer 2:GTTCAGGCCAACCATTCAGT