Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.086371 |
Chromosome: | scaffold 19 |
Location: | 29071 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
intergenic |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGAACAAAACCTTGGGGGGCCATCCTGAT |
Internal bar code: | TAGTTGTTACTAAAGAGGCAGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 612 |
LEAP-Seq percent confirming: | 99.095 |
LEAP-Seq n confirming: | 1314 |
LEAP-Seq n nonconfirming: | 12 |
LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GAAGTCCCGTAGAGTGCAGC |
Suggested primer 2: | GTTCAGGCCAACCATTCAGT |