| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.086376 |
| Chromosome: | chromosome 17 |
| Location: | 2490811 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g716050 | (1 of 1) PTHR20953:SF2 - PROTEIN SEEDLING PLASTID DEVELOPMENT 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCCATTGCAGCCCACCTGGGTTTGGTTT |
| Internal bar code: | CGCCCAGACTTGTTATGAGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 235 |
| LEAP-Seq percent confirming: | 43.8849 |
| LEAP-Seq n confirming: | 61 |
| LEAP-Seq n nonconfirming: | 78 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATGTAGCACTCCGTTCCC |
| Suggested primer 2: | TCACCACCAGAAATTGTCCA |