Insertion junction: LMJ.RY0402.086377_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.086377
Chromosome:chromosome 10
Location:3879575
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre10.g447700 (1 of 22) PTHR11017//PTHR11017:SF145 - LEUCINE-RICH REPEAT-CONTAINING PROTEIN // SUBFAMILY NOT NAMED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAGATGGGCGTGTGTACGTGCTTGTGTTTG
Internal bar code:TCCCGTCCCGCGGTGGCGCCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:107
LEAP-Seq percent confirming:85.1064
LEAP-Seq n confirming:320
LEAP-Seq n nonconfirming:56
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:AATAGGGTGCACGCAATAGG
Suggested primer 2:CTTGTAGCAATGTCGCTGGA