Insertion junction: LMJ.RY0402.086378_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.086378
Chromosome:chromosome 7
Location:1289824
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g321951 NRAMP2 Manganese/metal transporter, NRAMP homolog; (1 of 2) PTHR11706//PTHR11706:SF33 - MANGANESE TRANSPORTER // NATURAL RESISTANCE-ASSOCIATED MACROPHAGE PROTEIN 1 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGGTGAAGATGGGTGCGGTGTGGAAGCAG
Internal bar code:AGAGGCGCCACCGTCGCTTCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:231
LEAP-Seq percent confirming:74.7748
LEAP-Seq n confirming:1245
LEAP-Seq n nonconfirming:420
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:CCCTGGCTACATGATGTGTG
Suggested primer 2:GTGTATTGTGCGGTGAATCG