| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.086396 |
| Chromosome: | chromosome 10 |
| Location: | 2683926 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g437900 | SPL31 | (1 of 1) PTHR17204//PTHR17204:SF29 - PRE-MRNA PROCESSING PROTEIN PRP39-RELATED // SUBFAMILY NOT NAMED; Pre-mRNA splicing factor | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGCGGCGGCTCAGCGAGGTGCCCATCTA |
| Internal bar code: | GGGTGGGGGCCGATAGAGGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 582 |
| LEAP-Seq percent confirming: | 96.8399 |
| LEAP-Seq n confirming: | 3187 |
| LEAP-Seq n nonconfirming: | 104 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGCCTTACCGAAACCAGT |
| Suggested primer 2: | GATTTTGGTTGGGTTCCAGA |