| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.086399 |
| Chromosome: | chromosome 10 |
| Location: | 3929082 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g448250 | (1 of 33) PF13855 - Leucine rich repeat (LRR_8) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCGCGTATCATTGCTGAGGCAATAAGCAA |
| Internal bar code: | TGGCCAGGTGAACATTAGGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 475 |
| LEAP-Seq percent confirming: | 99.5074 |
| LEAP-Seq n confirming: | 202 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTTAACCTGTGTGGGGACT |
| Suggested primer 2: | AAAACAATCGAACGTACCGC |