| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.086420 |
| Chromosome: | chromosome 12 |
| Location: | 8842987 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g544900 | (1 of 1) PTHR16470:SF0 - UBIQUITIN DOMAIN-CONTAINING PROTEIN UBFD1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGTTGGGTTGGGGGATGGGATGGGTCGG |
| Internal bar code: | GATGTTGTCCCCCCTCCAGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 659 |
| LEAP-Seq percent confirming: | 98.2857 |
| LEAP-Seq n confirming: | 172 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGAAAGGGAATGGGTACAT |
| Suggested primer 2: | TAACCACCGCACTGTAACCA |