| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.086421 |
| Chromosome: | chromosome 16 |
| Location: | 5345041 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g681850 | (1 of 2) 2.4.2.9//2.7.1.48 - Uracil phosphoribosyltransferase / UMP pyrophosphorylase // Uridine kinase / Uridine monophosphokinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGAGCGCTGTCCCTAATGCCTGTATAGT |
| Internal bar code: | TATTGGGCCGTGTCTAATTAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 526 |
| LEAP-Seq percent confirming: | 99.1736 |
| LEAP-Seq n confirming: | 1920 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAACACAAAGTCGTTGGGC |
| Suggested primer 2: | AAGATCTACGTGGACACGGG |