| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.086440 |
| Chromosome: | chromosome 12 |
| Location: | 1644129 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g486209 | (1 of 1) K09533 - DnaJ homolog subfamily C member 13 (DNAJC13) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTTTCGATTCGGTTACATCCGTATGTTC |
| Internal bar code: | GTGCAGGAAGGGAAAGGGTAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 262 |
| LEAP-Seq percent confirming: | 91.4928 |
| LEAP-Seq n confirming: | 570 |
| LEAP-Seq n nonconfirming: | 53 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTGGCGGACTATGTGTAT |
| Suggested primer 2: | ACGGGTTCGCATGTTTTTAG |