| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.086460 |
| Chromosome: | chromosome 3 |
| Location: | 3564663 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g168100 | (1 of 781) IPR000104 - Antifreeze protein, type I | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCAAGTAAACTACACCTGTGTGGACTGTT |
| Internal bar code: | ATTAACTCGGAAGCCCGTGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 677 |
| LEAP-Seq percent confirming: | 98.7314 |
| LEAP-Seq n confirming: | 6382 |
| LEAP-Seq n nonconfirming: | 82 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTCCCTCCACCACATCAAC |
| Suggested primer 2: | CACTTGTTTGGCATGTCAGG |