Insertion junction: LMJ.RY0402.086464_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.086464
Chromosome:chromosome 4
Location:1238698
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre04.g215250 FAP26 Ankyrin Repeat Flagellar Associated Protein 26; (1 of 78) IPR002110//IPR020683 - Ankyrin repeat // Ankyrin repeat-containing domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTCTCAGGACAGGCGGCATACTTACCCGC
Internal bar code:CCAGTCGAGCAACATGCTACGG

Confirmation - LEAP-Seq

LEAP-Seq distance:282
LEAP-Seq percent confirming:98.509
LEAP-Seq n confirming:925
LEAP-Seq n nonconfirming:14
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GGCGACAAGCTTTGAAACTC
Suggested primer 2:TCTCCCTTCACGAGCAAAGT