| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.086486 |
| Chromosome: | chromosome 13 |
| Location: | 3419519 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g587250 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTACCATGTTTGCTGCGTGAGCACGGAGT |
| Internal bar code: | CGTGTTAGGCCGGAGTGCCGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 704 |
| LEAP-Seq percent confirming: | 99.141 |
| LEAP-Seq n confirming: | 2539 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGTTGTCGCTGATGGAACC |
| Suggested primer 2: | CTGAAGGCCGAGTTCTCATC |