| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.086496 |
| Chromosome: | chromosome 3 |
| Location: | 711191 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g146047 | (1 of 6) 3.1.1.1 - Carboxylesterase / Procaine esterase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGCCCTTTGCCAGGCTGAGCTTGCCGTT |
| Internal bar code: | CGCCCTTTTGGCTGCCTGAGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 173 |
| LEAP-Seq percent confirming: | 83.3333 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATAGCACGTGATAGGGCT |
| Suggested primer 2: | CAATCAAGTGCTGCGTCAGT |