Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.086496 |
Chromosome: | chromosome_3 |
Location: | 6018309 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g190950 | TUA1 | Alpha tubulin 1 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GACGAGGAAACGCAGTCAAGGGTACGAAAA |
Internal bar code: | GTTCAGACAAGAGCAATTGGGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 774 |
LEAP-Seq percent confirming: | 98.6799 |
LEAP-Seq n confirming: | 897 |
LEAP-Seq n nonconfirming: | 12 |
LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CACCAGGTTGGTCTGGAACT |
Suggested primer 2: | GATTTTCTCCGTCTCCTCCC |