| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.086550 |
| Chromosome: | chromosome 3 |
| Location: | 7316592 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g206800 | HXT1 | (1 of 1) PTHR23500//PTHR23500:SF118 - FAMILY NOT NAMED // PLASTIDIC GLUCOSE TRANSPORTER 4; Plastidic glucose transporter | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCTTCAAGGTAAAATGGGATTGGGGAGC |
| Internal bar code: | CTTTGTCTATTGTAGGAGATGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 237 |
| LEAP-Seq percent confirming: | 99.8365 |
| LEAP-Seq n confirming: | 1221 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGTTCCGCAAAATGGTCT |
| Suggested primer 2: | CCATACAGCCGCACATACAC |