Insertion junction: LMJ.RY0402.086550_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.086550
Chromosome:chromosome 3
Location:7316601
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g206800 HXT1 (1 of 1) PTHR23500//PTHR23500:SF118 - FAMILY NOT NAMED // PLASTIDIC GLUCOSE TRANSPORTER 4; Plastidic glucose transporter intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCAGGAGAACAGCACCCACGCACAGCAACC
Internal bar code:GTCGGAGACTTGGGCACGCTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:347
LEAP-Seq percent confirming:99.0515
LEAP-Seq n confirming:2924
LEAP-Seq n nonconfirming:28
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:GATGTTCCGCAAAATGGTCT
Suggested primer 2:CCATACAGCCGCACATACAC