| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.086564 |
| Chromosome: | chromosome 2 |
| Location: | 5046947 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g104550 | Paf1 complex subunit CDC73; (1 of 1) K15175 - parafibromin (CDC73) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCATGTAATCAGTTCAAGAATGTAAGCTAA |
| Internal bar code: | CGAAACCGGACTCCACGTCCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1121 |
| LEAP-Seq percent confirming: | 94.8052 |
| LEAP-Seq n confirming: | 146 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGACCCTTGCAAACCACTAA |
| Suggested primer 2: | GTCCAACACCTCCAGCATTT |