| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.086572 |
| Chromosome: | chromosome 3 |
| Location: | 1713513 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g153400 | (1 of 1) 3.3.2.9 - Microsomal epoxide hydrolase / Microsomal epoxide hydratase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTCCTGCGTGGCAGCATCTACTACCACCT |
| Internal bar code: | GGGACTTCGAGAAAATGGCGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 48 |
| LEAP-Seq percent confirming: | 79.1667 |
| LEAP-Seq n confirming: | 57 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCTCCTCTTTTGCACTGG |
| Suggested primer 2: | AATGTGATCCAAGCCCACTC |