| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.086578 |
| Chromosome: | chromosome 2 |
| Location: | 363244 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g075750 | (1 of 13) PF00646 - F-box domain (F-box) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGGCCGCTTCTCCAGCCTCACCAGCTTG |
| Internal bar code: | CCTTGATGCCCCAACGCCGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 95 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 46 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTCCGATCTACGCAAGAG |
| Suggested primer 2: | AATGACCAGGCTGTGGTAGC |