| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.086588 |
| Chromosome: | chromosome 16 |
| Location: | 734382 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g691050 | (1 of 1) PF08381 - Transcription factor regulating root and shoot growth via Pin3 (BRX) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTATTTGCAAATGCTCATGGACTGTGTCGC |
| Internal bar code: | TCAGGCTCGGAATGGTAGTGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 501 |
| LEAP-Seq percent confirming: | 96.6119 |
| LEAP-Seq n confirming: | 1939 |
| LEAP-Seq n nonconfirming: | 68 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGTACGTCAGCATGTCGC |
| Suggested primer 2: | GCCCTTACGTGGTAGCTGTC |