| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.086591 |
| Chromosome: | chromosome 12 |
| Location: | 8474926 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g548050 | CYG32 | (1 of 70) 4.6.1.2 - Guanylate cyclase / Guanylyl cyclase; Adenylate/guanylate cyclase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGCTGGTTGCTGGTTGGCGTGTTGAGTG |
| Internal bar code: | CACTCAGGCACTGGCAGAAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 445 |
| LEAP-Seq percent confirming: | 99.7833 |
| LEAP-Seq n confirming: | 4604 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGCCGTGTTATGAAACCA |
| Suggested primer 2: | CACGATTAGAGGCATCAGCA |