Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.086619 |
Chromosome: | chromosome_2 |
Location: | 5525286 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre02.g108450 | FAP280 | Flagellar Associated Protein, transcriptional coactivator-like | antisense | 5'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TGGAAGCTGCAGCGAGCACTAATCAAGTGA |
Internal bar code: | AGCGGTTAGTGCGGGATTGCAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 689 |
LEAP-Seq percent confirming: | 98.4 |
LEAP-Seq n confirming: | 2460 |
LEAP-Seq n nonconfirming: | 40 |
LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CAAAGTTGTCCCCTCCGTAA |
Suggested primer 2: | GAAACTTGATGTGTGCGTGG |