| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.086623 |
| Chromosome: | chromosome_3 |
| Location: | 2528661 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g160050 | FAP184 | Flagellar Associated Protein | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATGCAAAGATAGGCTGGATGTACAATGGGC |
| Internal bar code: | CCTGTTCCATGAGGAGGATTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 993 |
| LEAP-Seq percent confirming: | 97.929 |
| LEAP-Seq n confirming: | 662 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGGCGCGTTTATTGTATG |
| Suggested primer 2: | CAGGTTCAGAAGGAGAAGCG |