| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.086631 |
| Chromosome: | chromosome 14 |
| Location: | 2640092 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g626200 | P4H7,PFH7,PHX18 | Prolyl 4-hydroxylase 7; (1 of 14) K00472 - prolyl 4-hydroxylase (E1.14.11.2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCAAATGGAAGCACACAGCACAAGAGCAG |
| Internal bar code: | CGCTCGTGTGTCGGTCGCGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 587 |
| LEAP-Seq percent confirming: | 98.3696 |
| LEAP-Seq n confirming: | 543 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGATAACACAGCCTACCCG |
| Suggested primer 2: | GGCTACGCCTTCACTTCTTG |