| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.086639 |
| Chromosome: | chromosome 3 |
| Location: | 728174 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g146087 | (1 of 3) 1.14.11.33 - DNA oxidative demethylase / Alkylated DNA repair protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCGTCGTGTTAGGTTTGCAGCGCTGTGT |
| Internal bar code: | GTCAGTGATCTGGCGGGGTTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 525 |
| LEAP-Seq percent confirming: | 94.1476 |
| LEAP-Seq n confirming: | 370 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGAAGAAACAGCAGCAGCA |
| Suggested primer 2: | CACTCACTTGGACCCATCCT |