| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.086658 |
| Chromosome: | chromosome 14 |
| Location: | 3406341 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g630500 | TIE2 | (1 of 1) K15323 - tRNA-splicing endonuclease subunit Sen34 (TSEN34); tRNA-intron endonuclease | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGGGGAGTCAGGGAGTGTGGTGCGTGGC |
| Internal bar code: | CCAATGTGCCCAGCTTCTCTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 593 |
| LEAP-Seq percent confirming: | 91.4894 |
| LEAP-Seq n confirming: | 172 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACACACCGTTTCTTTCCC |
| Suggested primer 2: | TGTGTGTGTGTGTGTGTCCC |