| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.086665 |
| Chromosome: | chromosome 17 |
| Location: | 529086 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g699550 | (1 of 37) IPR008979 - Galactose-binding domain-like | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTATGCGGCGTGACGAGCCTGTTGCTGGAC |
| Internal bar code: | TTTGTAATATGCAAGGCGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 274 |
| LEAP-Seq percent confirming: | 99.5121 |
| LEAP-Seq n confirming: | 4895 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCACAGCAGCAAATCACAC |
| Suggested primer 2: | ACAACGCCCAAGGTGTCTAC |