| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.086686 |
| Chromosome: | chromosome 1 |
| Location: | 3132876 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g019500 | (1 of 2) PTHR22847//PTHR22847:SF431 - WD40 REPEAT PROTEIN // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACCCTTCCAGTGCTGCGCCGGCATGTCCT |
| Internal bar code: | CTAGGGCCTTAGGCAGGATTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 493 |
| LEAP-Seq percent confirming: | 98.021 |
| LEAP-Seq n confirming: | 2526 |
| LEAP-Seq n nonconfirming: | 51 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTTGAAGGCGTAAAGCAGC |
| Suggested primer 2: | AGCTGTGTGTGTTCCACCTG |