| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.086688 |
| Chromosome: | chromosome_8 |
| Location: | 1018044 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre08.g362050 | FAP49 | Flagellar Associated Protein, membrane-associated | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCCTATCCATGGAAAACCCCTCGACCCAGC |
| Internal bar code: | AGGTACCAGCTTTTTTCCTTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 428 |
| LEAP-Seq percent confirming: | 99.3141 |
| LEAP-Seq n confirming: | 2172 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTTCTAGCTACCCCGGCT |
| Suggested primer 2: | CTGCCTTACCACTGTCCCAT |