| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.086689 |
| Chromosome: | chromosome 6 |
| Location: | 8113684 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g304850 | (1 of 1) PF04424 - Protein of unknown function (DUF544) (DUF544) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCATTGATAAGTCACATAAACTTACTGGC |
| Internal bar code: | CCTTCTTCAGGCAAGTTCCCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 584 |
| LEAP-Seq percent confirming: | 99.6574 |
| LEAP-Seq n confirming: | 7855 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTAGGATTGAGGGGGAGG |
| Suggested primer 2: | GCCGAAGAGAACTGGAACTG |