| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.086704 |
| Chromosome: | chromosome 6 |
| Location: | 8234591 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g305850 | SMM20 | (1 of 2) PTHR12176:SF28 - S-ADENOSYL-L-METHIONINE-DEPENDENT METHYLTRANSFERASE-LIKE PROTEIN; S-adenosyl-L-methionine-dependent methyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCGCTGTACGCAACAGTAGCACCCCCAC |
| Internal bar code: | GCCGCGTCTAGTGGGTAGGAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 561 |
| LEAP-Seq percent confirming: | 97.7794 |
| LEAP-Seq n confirming: | 2598 |
| LEAP-Seq n nonconfirming: | 59 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCGCAGCCTCCTTATCAGT |
| Suggested primer 2: | CTTAGGCCTTTGACGCATGT |