| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.086712 |
| Chromosome: | chromosome 2 |
| Location: | 4722535 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g102050 | FAP328,CSE15 | Flagellar Associated Protein 328 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAAGCACGACGTTGTAGAGGCCTACCTGG |
| Internal bar code: | TGCTTCCTGGTGAGTGCACGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 898 |
| LEAP-Seq percent confirming: | 99.48 |
| LEAP-Seq n confirming: | 15306 |
| LEAP-Seq n nonconfirming: | 80 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACTGTTGTGAATTGGTGC |
| Suggested primer 2: | TGTGTAGCTTTTGCCACCAG |