| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.086718 |
| Chromosome: | chromosome 2 |
| Location: | 4674222 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g101500 | MRS1 | Mg2+ transporter protein; (1 of 3) K16075 - magnesium transporter (MRS2, MFM1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGGCGACCTGTCGCGACTGTCGTCCGGC |
| Internal bar code: | GCGGATGTGGACTAGTGTTACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 489 |
| LEAP-Seq percent confirming: | 98.412 |
| LEAP-Seq n confirming: | 2107 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCCATGCTCACAGAGTTCC |
| Suggested primer 2: | AGCGTGTGGACACTAATCCC |