| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.086718 |
| Chromosome: | chromosome 13 |
| Location: | 43686 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g562150 | (1 of 1) PTHR23305:SF12 - GTP-BINDING PROTEIN-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGACAGCCATGATCAAATGGGTGAGAATGG |
| Internal bar code: | CCTACTTTGAAGCATTGGCAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 840 |
| LEAP-Seq percent confirming: | 99.0385 |
| LEAP-Seq n confirming: | 721 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGCCCAAACTAAACCAAA |
| Suggested primer 2: | GTTCGCACCCGATTTACACT |