| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.086742 |
| Chromosome: | chromosome_9 |
| Location: | 3907176 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g392282 | DHC2 | Dynein heavy chain 2, inner arm dynein d (monomeric) | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AGGAGGTGCGAATACGTCACCTCGTCGCTC |
| Internal bar code: | GTGCGACCTCGGTTAATTGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1244 |
| LEAP-Seq percent confirming: | 99.526 |
| LEAP-Seq n confirming: | 9868 |
| LEAP-Seq n nonconfirming: | 47 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCGCTTCATGCTTCCTAGA |
| Suggested primer 2: | ACTCGTGTTTCCCCCTCTTT |