Insertion junction: LMJ.RY0402.086746_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.086746
Chromosome:chromosome 12
Location:4553277
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre12.g522300 (1 of 1) IPR001611//IPR002048//IPR003590//IPR008907//IPR011992 - Leucine-rich repeat // EF-hand domain // Leucine-rich repeat, ribonuclease inhibitor subtype // P25-alpha // EF-hand domain pair 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGCAGCACACCAGCACACCAGCGCTCACC
Internal bar code:GACCGCTCGCCAGTCGCGGTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:642
LEAP-Seq percent confirming:99.701
LEAP-Seq n confirming:667
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:ACGGATTGCATCACGTTGTA
Suggested primer 2:GAGGGCAACATCAAGACCAT