Insertion junction: LMJ.RY0402.086758_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.086758
Chromosome:chromosome 11
Location:1204182
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g467693 (1 of 5) PF07466 - Protein of unknown function (DUF1517) (DUF1517) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCTGATTGCCTCCCCACTCCACGTCACCA
Internal bar code:GCTAGATTTAGCGCTAGATTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:678
LEAP-Seq percent confirming:98.5816
LEAP-Seq n confirming:1668
LEAP-Seq n nonconfirming:24
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:CCGTGTGTGTGTGTGTGTGT
Suggested primer 2:AACCACAGGGAACTAGCCCT