| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.086772 |
| Chromosome: | chromosome 8 |
| Location: | 29430 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g358350 | OPR34,TDA1 | Translation factor for atpA | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGACACAAGCGCAACAGGAGGCGTCAGAC |
| Internal bar code: | TGATAAGGGTTGTTGAGGAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 577 |
| LEAP-Seq percent confirming: | 49.789 |
| LEAP-Seq n confirming: | 354 |
| LEAP-Seq n nonconfirming: | 357 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTAAGGTGTTTGCTGGCTG |
| Suggested primer 2: | GTAGGAACGGATTAACGGCA |