| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.086795 |
| Chromosome: | chromosome 16 |
| Location: | 858757 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g647901 | CSC8 | Chlamydomonadale-specific family C protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCCTTGGTAGTGGTTGTCGACGCAGCGC |
| Internal bar code: | GCTAATTTGGAGGTGTCCCCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 228 |
| LEAP-Seq percent confirming: | 98.5746 |
| LEAP-Seq n confirming: | 899 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAATGCCACAGTAGCCACC |
| Suggested primer 2: | TCGCCTGTTGTTTCTCTGTG |