Insertion junction: LMJ.RY0402.086817_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.086817
Chromosome:chromosome 2
Location:3777117
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre02.g095450 FKB16F,FKB9,FKB16-6 Peptidyl-prolyl cis-trans isomerase, FKBP-type; (1 of 1) PTHR10516:SF145 - PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FKBP42 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAGTGCCAACTGCGAACTGTCAACATGACA
Internal bar code:CAGTCACAGGAAGCGACCCAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:525
LEAP-Seq percent confirming:80.0
LEAP-Seq n confirming:448
LEAP-Seq n nonconfirming:112
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:AACTAAAGCAAGCCAAGCCA
Suggested primer 2:AGGTAGGTTGCTAGGTGCGA