| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.086821 |
| Chromosome: | chromosome 3 |
| Location: | 4121843 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g173250 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTATCAAGCATGGTGCTCGCATCGCCAG |
| Internal bar code: | CCAATCGCCCCGTGCGGCGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 145 |
| LEAP-Seq percent confirming: | 15.8921 |
| LEAP-Seq n confirming: | 742 |
| LEAP-Seq n nonconfirming: | 3927 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGGTCTTGCAGTCAATCA |
| Suggested primer 2: | AGCCCAGTCTTCTAGCACCA |